Duplication? No, I still want beer.

/Expression attenuation as a mechanism of robustness against gene duplication/, Proceedings of National Academy of Sciences, 2011. Budding yeast is still a fine system to do all kinds of imaginary experiments. To introduce a duplicated gene, yes, use single-copy centromeric plasmids (pCEN). To measure PPI interactions, yes, “use a protein-fragment complementation assay (PCA) based on he dihydrofolate reductase (DHFR) enzyme (DHFR-PCA)” (…) then the colony sizes can tell you about it. Also, of course, GFP and RNA-Seq are just a piece of cake.
Except for these magical experiments, here are a few more things:
1. Duplication of haploinsufficient genes seem to have higher chance of deleterious effects than haplosufficient genes.
2. Deleterious duplications have similar fractions for genes in and out of protein complexes.
3. Duplication may affect PPI, but tends to increase the strength or amount of PPIs of other subunits, however, the disturbance of PPI is not correlated with selection coefficient.
4. At the protein level, the amounts of protein are attenuated after duplication (but still slightly higher than the pre-duplication level?). Other subunits disturbed by a duplication may increase their protein abundance, consistent with increased amount of PPIs.
5. Attenuation can occur at the transcriptional level as well as the posttranscriptional level, and the latter is more frequent and requires the translation of mRNA.

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